Anadolu dağ kurbağalarının genetik çeşitliliğinin belirlenmesi


Thesis Type: Doctorate

Institution Of The Thesis: Recep Tayyip Erdogan University, Fen Bilimleri Enstitüsü, Turkey

Approval Date: 2017

Thesis Language: Turkish

Student: TUĞBA ERGÜL KALAYCI

Supervisor: Nurhayat Özdemir

Abstract:

Anatolian mountain frogs (Rana macrocnemis, Rana camerani, Rana holtzi and Rana

tavasensis) are distributed in some regions of the Caucasus, Iran, Iraq and Anatolia. The studies

that indicate the taxonomic status of species in the literature are mostly based on morphological

characteristics and there is no population genetic study of this group. In this study, we collected

132 specimens from 31 different populations of Anatolia and phylogenetic and population

genetic analyzes were performed based on base sequences of mtDNA (cyt b and COI) and

nDNA (RAG1 and POMC) and 6 different microsatellite locus. The high bootstrap and

posterior probability values of the Maximum parsimony, Bayesian inferences and Maximum

likelihood analyzes of both mtDNA gene regions (for cyt b: 99/1.0/72; MP / BI / MB, for COI:

100/1.0/100; MP / BI / MB) shows that the while R. tavasensis species separated from other

Anatolian mountain also, no genetic difference was obtained among other Anatolian mountain

frogs R. holtzi, R. camerani and R. macrocnemis species. No distinct genetic structuring has

been identified among the members of this group in terms of the nDNA genes examined. As a

result of the population genetic analysis, a similar genetic structure was observed among

Anatolian mountain frogs in terms of microsatellite DNA locus examined. In the light of the

basic information we have obtained, including R. tavasensis species; in some of our groups,

effective population size was not sufficient and significant bottlenecks were identified in some

locus. As a result of genetic distance analysis, no significant correlation between geographical

structure and genetic structure was found. As a result, Anatolian mountain frogs are divided into

two species in terms of mtDNA. In terms of nDNA, it has not shown a genetic structuring.