Journal Of Periodontology, vol.1, no.1, pp.1-8, 2020 (SCI-Expanded)
Background: Two main aims of this animal study were to inspect the possible effects of periodontitis on the structure and functions of the kidneys and thetherapeutic effectiveness of melatonin.
Methods: Twenty-four male Sprague-Dawley rats were randomly divided into three groups: control, experimental periodontitis (Ep), and Ep-Melatonin (Ep-Mel). Periodontitis was induced by placing 3.0 silk sutures sub-paramarginally around the cervix of right-left mandibular first molars and maintaining the sutures for 5 weeks. Then melatonin (10 mg/kg body weight/day, 14 days), and the vehicle was administered intraperitonally. Mandibular and kidney tissue samples were obtained following the euthanasia. Periodontal bone loss was measured via histological and micro-computed tomographic slices. On right kidney histopathological and immunohistochemical, and on the left kidney biochemical (MDA, GSH, OSI, TNF-α, IL-1β, MMP-8, MMP-9, and CtD levels) evaluations were carried out. Renal functional status was analyzed by levels of serum creatinine, urea, cystatin-C, and urea creatinine.
Results: Melatonin significantly restricted ligature induced periodontal bone loss (p<0,01) and suppressed the levels of proinflammatory cytokines (TNF-α and IL-1β), oxidative stress (MDA and OSI), and proteases (MMP-8, MMP-9, and CtD) that was significantly higher in the kidneys of the rats with periodontitis. (p<0,05) In addition, periodontitis related histological damages and apoptotic activity were also significantly lower in the Ep-Mel group. (p<0,05) However, the markers of renal function of the Ep group were detected slightly impaired in comparison with the control group (p>0.05); and the therapeutic activity of melatonin was limited. (p>0.05)
Melatonin restricts the periodontitis induced
inflammatory stress, apoptosis, and structural but not functional impairments.