Doppler ultrasonography (DUSG) is widely used for fetal evaluations. This study investigated the effects of new generation Doppler ultrasound application at different frequencies during pregnancy on postnatal renal development. Six pregnant female rats were divided into three groups. No procedure was performed on the first (control) group. In the second group, transabdominal DUSG was performed continuously for 15 min every day from the first day of gestation until birth. In the third group, DUSG was applied for 15 min every two days. Twenty-four male pups were sacrificed after 60 days. Renal tissues were then collected and subjected to biochemical, histopathological, and immunohistochemical evaluation. Malondialdehyde, glutathione, urea, Ca, K, and Cl levels increased in the DUSG groups compared to the control group (p < .05). Histopathologically, tubular damage increased in the DUSG groups compared to the control group (p < .05). Immunohistochemically, an increase was determined in Caspase-3 expression in the DUSG groups compared to the control group (p > .05). The DUSG groups also exhibited an increase in the superficial areas of the proximal and distal tubules, although the difference compared to the control group was not significant (p > .05). Multiple administrations of new generation DUSG to pregnant rats resulted in deleterious effects on the development of postnatal renal tissue. This shows that DUSG should be applied for as short a time as possible and that re-exposure should be avoided.