Materials with triboelectric properties are critical to boosting the efficiency of triboelectric nanogenerators (TENG) by gathering power from waste force and mechanical movement. In this study, we explored tin-oxide (SnO2) thin films with silver (Ag) seed layer properties for friction surfaces at TENGs. Investigation of the triboelectric behavior of semi-conductor/polymer coupling structured TENGs formed by thin films with and without Ag seed layer after heat treatment was explored by X-ray diffraction (XRD), electron microscopy (SEM), atomic force microscopy (AFM), and semi-logarithmic current-voltage measurements. Similar structural characterization studies were carried out in indium tin oxide coated polyethylene terephthalate film (PET/ITO) for the polymer friction layer. Semiconductor/polymer coupling structured TENG efficiency was evaluated in contact-separation mode and the output voltage-current was enhanced to 80 V, 4 μA, and 210 μW of output power calculated by depositing a SnO2 thin film on an Ag seed layer, respectively. Our findings offer a different perspective for synthesizing the semi-conductor/polymer coupling structured TENGs for promising applications in a range of application technologies.