Metformin and atorvastatin reduce adhesion formation in a rat uterine horn model


Yilmaz B., Aksakal O., Gungor T., Sirvan L., SÜT N., Kelekci S., ...More

REPRODUCTIVE BIOMEDICINE ONLINE, vol.18, no.3, pp.436-442, 2009 (SCI-Expanded) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 18 Issue: 3
  • Publication Date: 2009
  • Doi Number: 10.1016/s1472-6483(10)60106-x
  • Journal Name: REPRODUCTIVE BIOMEDICINE ONLINE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.436-442
  • Keywords: adhesion prevention, atorvastatin, metformin, rat, uterine horn model, PLASMINOGEN-ACTIVATOR INHIBITOR, POLYCYSTIC-OVARY-SYNDROME, L6 MUSCLE-CELLS, POSTOPERATIVE ADHESION, HYALURONATE/CARBOXYMETHYLCELLULOSE MEMBRANE, FIBRINOLYTIC-ACTIVITY, ENDOTHELIAL-CELLS, HYALURONIC-ACID, RISK-FACTORS, PREVENTION
  • Recep Tayyip Erdoğan University Affiliated: No

Abstract

The aim of the present study was to determine whether atorvastatin and metformin are effective in preventing adhesions in a rat uterine horn model. A total of 40 non-pregnant, female Wistar albino rats, weighing 180-210 g, were used as a model for post-operative adhesion formation. The rats were randomized into four groups after seven standard lesions were inflicted in each uterine horn and lower abdominal sidewall using bipolar cauterization. The rats were given atorvastatin 2.5 mg/kg/day, p.o. (10 rats), atorvastatin 30 mg/kg/day, p.o. (10 rats), metformin 50 mg/kg/day, p.o. (10 rats) and no treatment was applied in the control group (10 rats). The animals were killed 2 weeks later and adhesions were scored both clinically and pathologically by authors blinded to groups. One rat in the control group died before the end of the 2 week period. Total clinical adhesion scores regarding extent, severity and degree of adhesions and histopathological findings including inflammation and fibrosis were significantly lower in the metformin (P < 0.001 and P < 0.01, respectively) and atorvastatin 30 mg/kg/day (P < 0.001 and P < 0.01, respectively) groups when compared with control group. Metformin and atorvastatin are both effective for prevention of adhesion formation in a rat uterine horn model.