In this study, stochastic responses of a cable-stayed bridge subjected to the spatially varying earthquake ground motion are investigated by the finite element method taking into account soil-structure interaction (SSI) effects. The considered bridge in the analysis is Quincy Bay-view Bridge built on the Mississippi River in between 1983-1987 in Illinois, USA. The bridge is composed of two H-shaped concrete towers, double plane fan type cables and a composite concrete-steel girder deck. In order to determine the stochastic response of the bridge, a two-dimensional lumped masses model is considered. Incoherence, wave-passage and site response effects are taken into account for the spatially varying earthquake ground motion. Depending on variation in the earthquake motion, the response values of the cable-stayed bridge supported on firm, medium and soft foundation soil are obtained, separately. The effects of SSI on the stochastic response of the cable-stayed bridge are also investigated including foundation as a rigidly capped vertical pile groups. In this approach, piles closely grouped together beneath the towers are viewed as a single equivalent upright beam. The soil-pile interaction is linearly idealized as an upright beam on Winkler foundation model which is commonly used to study the response of single piles. A sufficient number of springs on the beam should be used along the length of the piles. The springs near the surface are usually the most important to characterize the response of the piles surrounded by the soil; thus a closer spacing may be used in that region. However, in generally springs are evenly spaced at about half the diameter of the pile. The results of the stochastic analysis with and without the SSI are compared each other while the bridge is under the sway of the spatially varying earthquake ground motion. Specifically, in case of rigid towers and soft soil condition, it is pointed out that the SSI should be significantly taken into account for the design of such bridges.