RELATIONSHIPS AMONG TURKISH VINCETOXICUM WOLF (APOCYNACEAE: ASCLEPIADOIDEAE) INFERRED FROM CPDNA TRNT– TRNL DATA


GÜVEN S. , MAKBUL S. , COSKUNCELEBI K.

3RD INTERNATIONAL CONFERENCE ON ENVIRONMENTAL SCIENCE AND TECHNOLOGY, Budapeşte, Hungary, 19 - 23 October 2017, vol.1, no.1, pp.94

  • Publication Type: Conference Paper / Full Text
  • Volume: 1
  • City: Budapeşte
  • Country: Hungary
  • Page Numbers: pp.94

Abstract

The present study aimed to explore phylogenetic relationships among Vincetoxicum Wolf distributed in Turkey based on cpDNA trnT–trnL sequences data. Total genomic DNAs were extracted by using silica gel-dried healthy leaves collected from natural habitats between 2012 and 2014 according to CTAB extraction protocol. Sequence analysis of PCR products were performed by Macrogen Inc. All the sequences were aligned with the Bioedit v.7.0 and then analyzed with MEGA v7 software. Average length and GC % content of trnT–trnL regions of the examined 20 samples were found to be 804 bp and 25.7 %, respectively. A data set of 804 bp belong to Vincetoxicum samples indicated that trnT– trnL regions contained 1 bp (% 0.12) parsimony informative sites, 2 bp (% 0.25) variable sites and 802 (% 99.75) conserved sites. Phylogenetic trees from both MP and ML analyses showed that all examined taxa fall into two distinct clades. While members of V. tmoleum Boiss. and V. canescens (Willd.) Decne were grouped in Clade I, the remaining members belong to examined Vincetoxicum taxa were clustured in Clade II. However present findings did not provide enough data to separate Vincetoxicum taxa at interspecific level. Acknowledgement: The authors extend their thanks to RTEUBAP (Project number 2015.53007.102.03.06) for the financial support. Keywords: Taxonomy, Trnt–Trnl, Turkey, Vincetoxicum.