Akademik Veri Yönetim Sistemi
CARBOHYDRATE RESEARCH, cilt.562, 2026 (SCI-Expanded, Scopus)
In this study, the phytochemical investigation of Scorzonera yildirimlii and S. zorkunensis led to the isolation and identification of eight new oligosaccharide constituents (1-8). Their structures were elucidated through comprehensive spectroscopic analysis (NMR, FT-IR, and mass spectrometry) and comparison with literature data. The structure of isolated compounds were O-a-D-Glcp-(1-* 2)-O-/3-D-Fruf-(3-* 1)-O-myo-inositol (1), O-a-D-Glcp-(1-* 2)-O-/3-D-Fruf-(3-* 1)-O-epi-inositol (2), O-/3-D-Fruf-(2-* 6)-O-a-D-Glcp-(1-* 1)-O-/3-D-Glcp (3), O-/3-D-Fruf-(2-* 6)-O-alpha-D-Glcp-(1-* 1)-O-/3-D-Galp (4), O-/3-D-Fruf-(2-* 6)-O-/3-D-Fruf-(2-* 6)-O-a-D-Glcp-(1-* 1)-O-/3-D-Glcp (5), O-/3-D-Fruf-(2-* 6)-[O-/3-D-Glcp-(1-* 4)]-O-a-D-Glcp-(1-* 1)-O-/3-D-Glcp (6), O-/3-D-Fruf-(2-* 6)-[O-/3-D-Glcp-(1-* 4)]-3-OCH3-O-a-D-Glcp-(1-* 1)-O-/3-D-Glcp (7), and O-/3-D-Fruf-(2-* 6)-[O-/3-D-Glcp-(1-* 4)]- O-a-D-Glcp-(1-* 4)-O-a-D-Glcp-(1-* 2)-O-/3-D-Fruf (8). The antimicrobial activities of all the isolated compounds (1-8) were investigated against six microorganisms. Compounds 2 and 3 were effective against Mycobacterium smegmatis at minimum inhibitory concentrations (MICs) of 3.13 mu g/mL and 3.44 mu g/mL, respectively, compared with streptomycin (MIC = 4.0 mu g/mL). The enzyme-inhibitory properties of the isolated compounds (1-8) were evaluated against tyrosinase and a-glucosidase. Among the tested compounds, 4 (39.78 +/- 10.29 %) exhibited inhibitory activity against tyrosinase, while compound 2 (68.04 +/- 4.68 %) exhibited inhibitory activity against alpha-glucosidase. In silico studies of 1-8 showed the best predicted binding affinities for the Trehalose-LpqY complex in the range of-11.088 kcal/mol to-14.297 kcal/mol, compared to streptomycin (-6.638 kcal/mol). Compounds 1-8 exhibited almost no cytotoxic activity against L-929 cells, further supporting their potential for therapeutic use.