Chemical analysis and biological activity of the essential oils and extracts of two liverwort species growing in Turkey


Celik G., Sahin H., BALTAŞ N., BATAN N., ALPAY KARAOĞLU Ş., YAYLI N.

BOTANICA SERBICA, vol.47, no.1, pp.31-40, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 47 Issue: 1
  • Publication Date: 2023
  • Doi Number: 10.2298/botserb2301031c
  • Journal Name: BOTANICA SERBICA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, Central & Eastern European Academic Source (CEEAS), Directory of Open Access Journals
  • Page Numbers: pp.31-40
  • Keywords: Diplophyllumtaxifolium Diplophyllum, albicans oil composition GC, FID, MS biological evaluation phenolic, constituents HPLC-UV, ANTIMICROBIAL ACTIVITY, VOLATILE CONSTITUENTS, XANTHINE-OXIDASE, ANTIOXIDANT ACTIVITIES, INHIBITION, MOSSES, SPME, SESQUITERPENOIDS, DIPLOPHYLLUM, BRYOPHYTES
  • Recep Tayyip Erdoğan University Affiliated: Yes

Abstract

The aim of this study was to investigate the chemical composition of the essential oils (EOs) from two Diplophyllum species and to evaluate their bioactivity potential [antimicrobial, antioxidant, anti-urease, anti-xanthine oxidase (XO)], and pheno-lic compounds. The analysis of Diplophyllum albicans and Diplophyllum taxifolium permitted the identification of 62 components, comprising >= 99.6% of the total EO composition. The major components found in these liverwort species were (3-patchoulene, (3-santalene, (3-himachalene, and cubebol. The antimicrobial assays showed that the solvent extracts (n-hexane and methanol) from these liverwort species exhibited weak to moderate antimicrobial activity. In addition, the metha-nol extracts of these liverwort species also exhibited moderate to high antioxidant potential. The enzyme inhibitory effects of the species were determined using ure-ase and XO for the methanol extracts. Generally, the methanol extracts of D. al-bicans and D. taxifolium exhibited powerful urease inhibition with IC50 values of 9.711 +/- 0.058 and 6.304 +/- 0.099 mu g/mL, respectively, compared to the IC50 value of 26.124 +/- 0.077 mu g/mL for the standard (acetohydroxamic acid). Moreover, the HPLC-UV detection method showed that the analysed methanol extracts of these liverwort species contained only catechin and benzoic acid. These findings suggest that the analysed liverwort species possess antioxidant and urease inhibition, thus indicating the potential to explore new bioactive molecules.