Identification of potential entomopathogenic bacteria in the culturable bacterial flora of the Brown Marmorated Stink Bug (<i>Halyomorpha halys</i>, Stål, 1885) (Hemiptera: Pentatomidae)

Gencer D., BAYRAMOĞLU Z., Aygun K., DEMİR İ.

JOURNAL OF PLANT DISEASES AND PROTECTION, cilt.133, sa.1, 2025 (SCI-Expanded, Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 133 Sayı: 1
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1007/s41348-025-01195-x
  • Dergi Adı: JOURNAL OF PLANT DISEASES AND PROTECTION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Geobase
  • Recep Tayyip Erdoğan Üniversitesi Adresli: Evet

Özet

The Brown Marmorated Stink Bug (Halyomorpha halys) has become a major invasive agricultural pest in T & uuml;rkiye, particularly threatening hazelnut production in the Black Sea Region. This study aimed to isolate and identify culturable bacterial flora from H. halys populations in T & uuml;rkiye and assess their potential as biological control agents. A total of nine bacterial isolates were obtained from diseased individuals and identified using morphological, biochemical, and 16 S rRNA gene sequencing analyses. A total of nine bacterial isolates were obtained and identified as belonging to six genera: Bacillus, Staphylococcus, Mammaliicoccus, Enterobacter, Acinetobacter, and Lysinibacillus. Insecticidal bioassays revealed that all isolates exhibited varying levels of pathogenicity against H. halys, with Bacillus thuringiensis (Hh18) causing 100% mortality in nymphs and 66% in adults at 1 x 10(7) CFU/mL after 10 days. Staphylococcus aureus and Acinetobacter sp. caused 86% mortality in nymphs, while Mammaliicoccus sciuri achieved 53% mortality in adults. These quantitative results provide strong evidence for the entomopathogenic potential of native bacterial isolates against H. halys.