Identification of potential entomopathogenic bacteria in the culturable bacterial flora of the Brown Marmorated Stink Bug (<i>Halyomorpha halys</i>, Stål, 1885) (Hemiptera: Pentatomidae)
JOURNAL OF PLANT DISEASES AND PROTECTION, cilt.133, sa.1, 2025 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 133 Sayı: 1
- Basım Tarihi: 2025
- Doi Numarası: 10.1007/s41348-025-01195-x
- Dergi Adı: JOURNAL OF PLANT DISEASES AND PROTECTION
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Geobase
- Recep Tayyip Erdoğan Üniversitesi Adresli: Evet
Özet
The Brown Marmorated Stink Bug (Halyomorpha halys) has become a major invasive agricultural pest in T & uuml;rkiye, particularly threatening hazelnut production in the Black Sea Region. This study aimed to isolate and identify culturable bacterial flora from H. halys populations in T & uuml;rkiye and assess their potential as biological control agents. A total of nine bacterial isolates were obtained from diseased individuals and identified using morphological, biochemical, and 16 S rRNA gene sequencing analyses. A total of nine bacterial isolates were obtained and identified as belonging to six genera: Bacillus, Staphylococcus, Mammaliicoccus, Enterobacter, Acinetobacter, and Lysinibacillus. Insecticidal bioassays revealed that all isolates exhibited varying levels of pathogenicity against H. halys, with Bacillus thuringiensis (Hh18) causing 100% mortality in nymphs and 66% in adults at 1 x 10(7) CFU/mL after 10 days. Staphylococcus aureus and Acinetobacter sp. caused 86% mortality in nymphs, while Mammaliicoccus sciuri achieved 53% mortality in adults. These quantitative results provide strong evidence for the entomopathogenic potential of native bacterial isolates against H. halys.