The volatile components of essential oil (EO), SPME, and SPME of solvent extracts (n-hexane, methanol, and water) obtained from fresh Serapias orientalis subsp. orientalis(Soo) were analyzed by GC-FID/MS. EO of Soo gave 11 compounds in the percentage of 99.97%; capronaldehyde (37.01%), 2-(E)-hexenal (23.19%), and n-nonanal (19.05%) were found to be major constituents. SPME GC-FID/MS analyses of fresh plant and solvent extracts of Soo revealed 7, 12, 7, and 4 compounds within the range of 99.7% to 99.9%. Limonene (76.5%, 41.7%, and 61.3%) was the major compound in SPMEs of the n-hexane and methanol extracts. alpha-Methoxy-p-cresol (52.9%) was the main component in its water extract. The antimicrobial activity of EO and the solvent extracts of Soo were screened against 9microorganisms. EO showed the best activity against Mycobacterium smegmatis, with 79.5 mu g/mL, MIC value. The n-hexane, methanol, and water extracts were the most active against the Staphylococcus aureus within the range of 81.25-125.0 mu g/mL (MIC). IC50 values for the lipase enzyme inhibitory activity of EO and solvent extracts (n-hexane, methanol, and water) were determined to be 59.87 mu g/mL, 64.03 mu g/mL, 101.91 mu g/mL, and 121.24 mu g/mL, respectively.