Melatonin improves periodontitis induced kidney damages by decreasing inflammatory stress and apoptosis in rats

Köse O., Kurt Bayrakdar S. , Ünver B. , Altın A. , Akyıldız K., Mercantepe T. , ...More

Journal Of Periodontology, vol.1, no.1, pp.1-8, 2020 (Journal Indexed in SCI)

  • Publication Type: Article / Article
  • Volume: 1 Issue: 1
  • Publication Date: 2020
  • Title of Journal : Journal Of Periodontology
  • Page Numbers: pp.1-8


Background: Two main aims of this animal study were to inspect the possible effects of periodontitis on the structure and functions of the kidneys and the therapeutic effectiveness of melatonin.

Methods: Twenty-four male Sprague-Dawley rats were randomly divided into three groups: control, experimental periodontitis (Ep), and Ep-Melatonin (Ep-Mel). Periodontitis was induced by placing 3.0 silk sutures sub-paramarginally around the cervix of right-left mandibular first molars and maintaining the sutures for 5 weeks. Then melatonin (10 mg/kg body weight/day, 14 days), and the vehicle was administered intraperitonally. Mandibular and kidney tissue samples were obtained following the euthanasia. Periodontal bone loss was measured via histological and micro-computed tomographic slices. On right kidney histopathological and immunohistochemical, and on the left kidney biochemical (MDA, GSH, OSI, TNF-α, IL-1β, MMP-8, MMP-9, and CtD levels) evaluations were carried out. Renal functional status was analyzed by levels of serum creatinine, urea, cystatin-C, and urea creatinine.

Results: Melatonin significantly restricted ligature induced periodontal bone loss (p<0,01) and suppressed the levels of proinflammatory cytokines (TNF-α and IL-1β), oxidative stress (MDA and OSI), and proteases (MMP-8, MMP-9, and CtD) that was significantly higher in the kidneys of the rats with periodontitis. (p<0,05) In addition, periodontitis related histological damages and apoptotic activity were also significantly lower in the Ep-Mel group. (p<0,05) However, the markers of renal function of the Ep group were detected slightly impaired in comparison with the control group (p>0.05); and the therapeutic activity of melatonin was limited.  (p>0.05)

Conclusion: Melatonin restricts the periodontitis induced inflammatory stress, apoptosis, and structural but not functional impairments.