Seawater-derived ureolytic <i>Vibrio</i> sp. strain F-11 as a microbial agent for calcium carbonate biomineralization
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, vol.119, no.6, 2026 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 119 Issue: 6
- Publication Date: 2026
- Doi Number: 10.1007/s10482-026-02329-3
- Journal Name: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, EMBASE, Environment Index
- Recep Tayyip Erdoğan University Affiliated: Yes
Abstract
This study investigates the strain F-11, a ureolytic Vibrio isolated from seawater, and its potential for microbially induced calcium carbonate precipitation (MICP). Based on morphological and biochemical characteristics together with 16S rRNA gene sequence analysis, strain F-11 showed the high-est similarity to Vibrio alginolyticus (96.79%) and was therefore conservatively designated as Vibrio sp. F-11. Urease activity measured by Nessler-based ammonium assay was examined at different temperatures (24, 30, 36 degrees C) and pH values (6, 8, 10). Maximum ureolytic activity was observed between days 3 and 5at 30 degrees C and pH 8, reaching approximately 0.58 and 0.57 mu mol min-1 mg-1 protein, respectively, whereas suboptimal conditions significantly reduced activity. Calcium carbonate precipitation occurred only on urea-CaCl