Seawater-derived ureolytic <i>Vibrio</i> sp. strain F-11 as a microbial agent for calcium carbonate biomineralization

UZUN YAYLACI, ECREN; KARA, AYŞE; GÜLPINAR SEKBAN, DEMET; YAYLACI, MURAT

doi:10.1007/s10482-026-02329-3

Seawater-derived ureolytic <i>Vibrio</i> sp. strain F-11 as a microbial agent for calcium carbonate biomineralization

UZUN YAYLACI E., KARA A., GÜLPINAR SEKBAN D. Ü., YAYLACI M.

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, cilt.119, sa.6, 2026 (SCI-Expanded, Scopus)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 119 Sayı: 6
Basım Tarihi: 2026
Doi Numarası: 10.1007/s10482-026-02329-3
Dergi Adı: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, EMBASE, Environment Index
Recep Tayyip Erdoğan Üniversitesi Adresli: Evet

Özet

This study investigates the strain F-11, a ureolytic Vibrio isolated from seawater, and its potential for microbially induced calcium carbonate precipitation (MICP). Based on morphological and biochemical characteristics together with 16S rRNA gene sequence analysis, strain F-11 showed the high-est similarity to Vibrio alginolyticus (96.79%) and was therefore conservatively designated as Vibrio sp. F-11. Urease activity measured by Nessler-based ammonium assay was examined at different temperatures (24, 30, 36 degrees C) and pH values (6, 8, 10). Maximum ureolytic activity was observed between days 3 and 5at 30 degrees C and pH 8, reaching approximately 0.58 and 0.57 mu mol min^{^-1} mg^{^-1} protein, respectively, whereas suboptimal conditions significantly reduced activity. Calcium carbonate precipitation occurred only on urea-CaCl2 agar at pH 8; here, opaque white mineral deposits accumulated around bacterial colonies. Microscopic observations revealed rosette-like and spherical CaCO3 crystals, while SEM images revealed compact, spherical precipitates. EDS spectra dominated by Ca, C, and O peaks confirmed the formation of CaCO3 phases. In general, Vibrio sp. strain F-11 demonstrated effective biomineralization capacity and represents a promising MICP agent for future marine and cementitious biotechnological applications.