Scorzonera papposa, a member of the Scorzonera genus, is not investigated deeply regarding its volatile oil composition and seconder metabolite content in Turkey. In our study we were able to distinguish Scorzonera papposa plant parts regarding their chemical content. 56 different volatile oil components could be detected in different plant parts of this species using GC-MS analysis. Further, investigated plant material was screened using 13 bioactive phenolics namely, Apigenin, Caffeic, Catechin, Chlorogenic acid, Gallocatechin, Isoquercitrin, Luteolin, p-Coumaric acid, Quercetin, Quercitrin, Rosmarinic acid, Rutin and Thymol. Volatile oil analysis of different parts of S. papposa revealed huge variation regarding their essential oil components from which a lot of could be used in pharmaceutical investigations. The highest amounts were detected with 16,78% for Methyl palmitate in root, 30.44 % for Phytone in stem, 40.17 % for Phytone in leaf and and 31.84 % for Phytol in seed. Catechin, Chlorogenic acid, Hyperoside and Avicularin coud be deteceted in highest amount in all plant parts. Apigenin and Neochlorogenic acid was present only in stem parts. Important phenolic compounds as Caffeic acid, Eepicatechin, Gallocatechin, Isoquercitrin, Luteolin, p-Coumaric acid, Quercetin, Rosmarinic acid, Rutin and Thymol were determined in this Scorzonera species.