Development Of Electrochemical Cytosensor Using U-87 MG Cells on Collagen IV-Coated Electrospun PAN Nanofibers on ITO Surface

Kurt Ş., Doğru T., Bal Altuntaş D., Sevim Nalkıran H., Koçak Ç., Aslan S., ...Daha Fazla

18th Nanoscience and Nanotechnology Conference, İstanbul, Türkiye, 26 - 28 Ağustos 2024, ss.120

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: İstanbul
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.120
  • Recep Tayyip Erdoğan Üniversitesi Adresli: Evet

Özet

Nanobiotechnology is essential in advancing cancer research. Glioblastoma is the most aggressive form

of brain tumor in adults. In recent years, nanostructured materials have attracted considerable attention

due to their unique properties resulting from their size-dependent structural/electrical/optical behaviors,

and they have become appealing materials in many areas such as, cancer research, tissue engineering,

drug transfer and biosensor . Nanofibres offer distinct advantages in addressing complex problems that

are difficult to overcome in macro dimensions. Their unique physical and chemical properties in nano

dimensions provide solutions that are not achievable through traditional methods. In this study,

electrospun PAN and PAN/C500 nano-fibers s on ITO (Indium Tin Oxide) coated glass surface were

prepared and coated with collagen IV to establish a 3D glioblastoma tumor model using U-87 MG cells.

The cells in the model were evaluated morphologically by fluorescence microscopy and

electrochemical measurements were recorded. Electrochemical measurements were taken in addition

to fluorescence microscopy evaluation of the morphological characteristics of the cells in the threedimensional

(3D) model.