Akademik Veri Yönetim Sistemi
4 th EURASIA BIOCHEMICAL APPROACHES & TECHNOLOGIES (EBAT) CONGRESS, Antalya, Türkiye, 3 - 06 Kasım 2022, ss.74
In this study, it was aimed to investigate as the effect of some compounds on the acetylcholinesterases
(ACHE, EC3.1.1.7) purified from the nymphs and adults of Ricania simulans(Walker, 1851) (Hemiptera:
Ricaniidae) and to struggle this pest through the enzyme inhibition. ACHE plays an important role in
neurotransmission by hydrolyzing the neurotransmitter acetylcholine into choline and acetate and is
the target site of many insecticides for the struggle.1 The ACHEs were purified from the adults and
nymphs of R. simulans by the edrophonium-Sepharose affinity chromatography.3
In inhibition studies,
specific inhibitors of acetylcholinesterase, tacrine and edrophonium chloride, and newly synthesized
compounds were used. In the studies with tacrine and edrophonium chloride, IC50 values were
determined as 18.0±0.3 and 1.2±0.4 µM for adults, 2.4±0.3 and 0.6±0.09 µM for nymphs, respectively.
In the inhibition studies with newly having been synthesized compounds, it was determined that the
IC50 value of the compound [Zn(HL)(bipy)(OClO3)], which is the most effective in adults of R. simulans
acetylcholinesterase, was 3.2±0.8 µM, and the IC50 value of [Ni(HL)(bipy)(OClO3)] compound, which is
the most effective for nymphs, was 4.6±0.8 µM. As a result, it has been shown that these complexes
can be used potentially as metal-based insecticides in the agricultural struggle for R. simulans.