Volatile constituents of the essential oil which was obtained by hydrodistillation (HD) in a Clevengertype apparatus from the air-dried Salvia staminea Montbret & Aucher ex Bentham (Lamiaceae) collected from Bayburt (North East Part of Black Sea Reagan of Turkey), were analyzed by GC-FID and GC-MS. Additionally, volatiles for the whole part of S. staminea were analyzed by two different methods which are headspace (HS)-GCFID/MS and headspace solid-phase micro extraction (HS-SPME)-GC-FID/MS. As results of this study, thirty compounds from hydrodistillation, fifteen constituents from HS-SPME and two components from headspace of S. staminea were identified with GC-FID/MS. The major compounds identified in the essential oil, SPME and HS of S. staminea were linalyl acetate (23.30%, 85.07%, and 87.55%) and linalool (22.05%, 9.02%, and 12.44%), respectively. The antimicrobial activities of the essential oil of S. staminea were screened against seven microorganisms (Escherichia coli, Yersinia pseudotuberculosis, Staphylococcus aureus, Enterococcus faecalis, Bacillus cereus, Listeria monocytogenes, and Candida albican) and showed good antimicrobial activity against Gram-positive bacteria which is consistent with the literature with the other types of Salvia species. The essential oil of S. staminea showed good antioxidant activity with IC50 60.4 mu g/mL.