Akademik Veri Yönetim Sistemi
SOUTH AFRICAN JOURNAL OF BOTANY, cilt.160, ss.428-436, 2023 (SCI-Expanded)
Scab disease of apple caused by Venturia inaequalis results in significant economic losses in the apple industry throughout the apple growing regions of world. In present study, we investigated the response of apple cv. Prima against the V. inaequalis by examining the levels of antioxidants, reactive oxygen species (ROS) metabolism, and gene expression of the phenylpropanoid pathway. We found that the inoculated plant showed a significant increase in metabolites and enzyme activities involved in ROS metabolism. There was a considerable rise in antioxidants (metabolites) like apigenin, p-coumaric acid, (+) -catechin, epicatechin, quercetin, phloridzin, rutin, ferulic acid, and sinapic acid in the inoculated plant from 28 to 108.84 (g-1 FW). Superoxide dismutase (SOD) activity increased from 8.22 to 11.61 u/gm whereas catalase (CAT) activity increased from 22. 08 to 48.62 u/gm and peroxidase (POD) activity increased from 60.67 to 105.05 u/gm in treated. Gene expression was enhanced by 8.92, 19.92, 27.44, and 32.45 folds with respect to control at different stages of inoculation. The expression of genes in the phenylpropanoid pathway was also highly upregulated in response to the pathogen. The plant's defense response triggered a hypersensitive response (HR), which limited the growth of the pathogen V. inaequalis. The results suggest that the synthesis and accumulation of polyphenols play an essential role in the plant's defense response against the pathogen, and some components of the phenylpropanoid pathway may exhibit broad-spectrum disease resistance without yield penalties. & COPY; 2023 SAAB. Published by Elsevier B.V. All rights reserved.