Effect of Etching Protocols on the Bonding Performance of a HEMA-Free Two-Step Universal Adhesive to Caries-Affected Dentin


BiEce M., BEDİR F., EyiibogEu G. B., SERİN KALAY T., KARADAŞ M.

JOURNAL OF ADHESIVE DENTISTRY, cilt.28, sa.1, ss.123-132, 2026 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 1
  • Basım Tarihi: 2026
  • Doi Numarası: 10.3290/j.jad.c_2785
  • Dergi Adı: JOURNAL OF ADHESIVE DENTISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, MEDLINE, Directory of Open Access Journals, Biomedical Reference Collection: Corporate Edition (EBSCO)
  • Sayfa Sayıları: ss.123-132
  • Recep Tayyip Erdoğan Üniversitesi Adresli: Evet

Özet

Purpose: To assess the bonding performance of a two-step universal adhesive applied in etch-and-rinse and self-etch protocols to caries-affected dentin. Methods and Materials: Mid-coronal dentin surfaces were obtained from extracted human molars and assigned to either sound or caries-affected dentin groups. Specimens were randomly distributed into 12 subgroups (n = 10) based on adhesive type (G2-Bond Universal, Gluma Bond Universal, Clearfil S3 Bond Universal), bonding protocol (self-etch or etch-and-rinse), and evaluation period (24 h or aging). Resin composite blocks were fabricated and subjected to shear bond strength testing after 24 h or 10,000 thermocycles. Fourier-transform infrared spectroscopy was employed to measure the degree of conversion of each adhesive. Statistical analyses included ANOVA, Weibull analysis, and Kruskal-Wallis tests (a = 0.05). Results: Adhesive type and bonding protocol significantly influenced bond strength (p < 0.001), whereas substrate condition and thermocycling had no significant impact (p > 0.05). After aging, Clearfil S3 Bond Universal in the self-etch protocol presented considerably lower bond strength to caries-affected dentin compared with G2-Bond Universal and Gluma Bond Universal, with no significant difference between the latter two. After aging, Gluma Bond Universal and G2-Bond Universal revealed significantly greater bond strength to caries-affected dentin when applied in the self-etch protocol than in the etch-and-rinse protocol. No significant differences were detected among adhesives when the etch-and-rinse protocol was used, regardless of testing time and substrate. Conclusions: Phosphoric acid pre-etching negatively affected the bonding performance of Gluma Bond Universal and G2-Bond Universal to caries-affected dentin.