Isolation of Arbuscular Mycorrhizal Fungi (Amf) in Tea Plant Rhizosphere (Camellia Sinensis), Susceptibility of Potential Disease-Causing Isolates against to the Biocontrol (Trichoderma spp.) Agents

Alpay Karaoğlu Ş., Suyabatmaz Ş., Bozdeveci A.

International Tea Congress, Lenkeran State University, Lankaran, Azerbaijan, 13 - 15 May 2022, pp.193-194

  • Publication Type: Conference Paper / Summary Text
  • City: Lankaran
  • Country: Azerbaijan
  • Page Numbers: pp.193-194
  • Recep Tayyip Erdoğan University Affiliated: Yes


Tea (Camellia sinensis) is a perennial herb cultivated globally in 58 countries spanning all
five continents. Turkey ranks seventh in the world in terms of the width of tea agricultural
lands and provides employment in the sector for more than 200 thousand producers. Global
warming and long-term uniform chemical fertilizer applications in agricultural areas cause
deterioration in the soil ecosystem. When the soil microbial flora balance is disturbed, fungi,
which are the most resistant microorganisms to environmental conditions, invade the
environment, spread rapidly, and cause serious fungal plant diseases. This study, it was aimed
to isolate fungal species in the rhizosphere of dried tea seedlings taken from two different
gardens fertilized with chemical and organic fertilizers. Different parts of the plants (root,
root collar, stem, and leaves) were examined by macroscopic and cultural methods.
It was determined that the bacterial population and diversity of the soil and plant in the
diseased sample taken from the soil in which chemical fertilizers were used was extremely
reduced, and the fungal flora was still at a level that could be considered rich.
Bacillus sp.
common bacterium,
Penicillium sp. as common fungal genuses and Fusarium, Gliocladium,
Alternaria and Pestalotiopsis were identified as the plant diseases causative
genuses. It was observed that the disease-causing drying was Daiback and Collar cancer
(Canker). It was determined that there was a large number of bacterial and fungal diversity in
the rhizosphere of the diseased plant taken from the soil fertilized with organic fertilizer.
Isolated fungi were subjected to a leaf pathogenicity test. As potential disease factors;
Fusarium, Paecilomyces, Botrytis, Gliocladium, and Colletotrichum were identified. A dual
culture test was performed with
Trichoderma spp strains with strong biocontrol activity. An
isolate of
Fusarium sp. that was resistant to all tested biocontrol agents was identified.
Keywords: Arbuscular Mycorrhizal Fungi, Camellia sinensis, Daiback and Collar cancer,
Trichoderma sp.