Detection, isolation and identification of Mosquito Origin Bacillus sp. and development of biological control materials

9th International Entomopathogens and Microbial Control Congress, Trabzon, Türkiye, 28 - 31 Ekim 2024, ss.92, (Özet Bildiri)

Mosquitoes are globally recognized pests with significant impacts on ecosystems and human

health. Despite numerous studies focused on biological and genetic approaches to mosquito

control, these insects continue to be a problem today. Bacillus thuringiensis (Bt) is a soiloriginated

bacteria that has been used as a bacterial biopesticide, particularly effective on

larvae, and is widely used in the control of mosquitoes. It remains a key tool in mosquito

management efforts. Ongoing research seeks to discover more effective Bt isolates for mosquito

control, a priority within the scientific community. In this study, 18 isolates of the genus

Bacillus were obtained from mosquitoes of the genera Aedes (1), Anopheles (2), and Culex (15),

which were collected from approximately 15 distinct regions in Turkey and categorized based

on their morphological characteristics. The 16S rRNA gene regions of the isolates were

amplified and performed to sequence analysis, identifying several species including B.

licheniformis, B. thuringiensis, B. pumilus, B. subtilis, Brevibacillus sp., B. amyloliquefaciens,

B. atrophaeus, B. velezensis, B. paramycoides, B. zhangzhouensis, and B. siamensis. Notably,

Bt isolate has been identified from the Anopheles mosquito genus so far. Further biotesting of

the identified isolates against mosquitoes is ongoing.