Determination of Tolerance of Halfeti Rose (Rosa odorata cv. 'Louis XIV') Under In vitro Conditions
11th International Molecular Biology andBiotechnology Congress, Adana, Türkiye, 10 - 12 Kasım 2022, ss.74-83, (Tam Metin Bildiri)
- Yayın Türü: Bildiri / Tam Metin Bildiri
- Basıldığı Şehir: Adana
- Basıldığı Ülke: Türkiye
- Sayfa Sayıları: ss.74-83
- Recep Tayyip Erdoğan Üniversitesi Adresli: Hayır
Özet
Roses are propagated by vegetative methods. However, there are some problems in the reproduction of some species with traditional methods. The features that make propagation by tissue culture method attractive are the rate of spread, high plant quality standards and the production of disease-free plants. It is also an effective method for propagating a large number of single-type plants. Tissue culture techniques have started to be used in rose cultivation due to factors such as rapid growth and regeneration. In this context, in vitro techniques are applied using a small amount of source material in order to support this potential in plant reproduction. Rose of Halfeti (Rosa odorata 'Louis XIV') has been a popular plant for Turkiye recently. In this direction, it was qualified as a product identified with its location and received a 'Geographical Indication'. In the study, the appropriate sterilization protocol was determined for the plant in question. At the same time, the effects of different cytokinin and auxin concentrations on shoot formation, proliferation and rooting stages were investigated. Shoot explants were transfered to MS basal media containing 30 g l-1 sucrose supplemented with different concentrations of BAP and 0.5 mg l-l GA3. Significant success has been achieved in media containing 1.5 mg l-1 BAP. Half MS medium supplemented with different concentrations of IBA and NAA was applied during the rooting stage.