Three New Potential Cargo Microorganisms With Isolated From Honeybees in Turkey

Alpay Karaoğlu Ş., Suyabatmaz Ş., Bozdeveci A., Akpınar R., Sevim E., Sevim A.

2nd INTERNATIONAL SYMPOSIUM ON BIODIVERSITY RESEARCH, Rize, Turkey, 18 - 20 November 2020, vol.2, no.2, pp.304-305

  • Publication Type: Conference Paper / Summary Text
  • Volume: 2
  • City: Rize
  • Country: Turkey
  • Page Numbers: pp.304-305
  • Recep Tayyip Erdoğan University Affiliated: Yes


ABSTRACT In this study, it was aimed to identify the swarming bacteria (E2-1A, E21-1A and E176-1B) isolated from three bee samples taken from different geographical regions by traditional and molecular methods. It is nown that these swarming bacteria are capable of information processing, containing an exceptionally high number of communication-related genes. This means a very high "Bacterial Social Intelligence Coefficient (IQ) Score". That is why bacterial social intelligence can be estimated by their ability to communicate, sense their environment and adapt accordingly. In addition these microorganisms known as Cargo Microorganisms. The honeybee samples were first surface sterilized, disintegrated in saline water, kept at 80 °C for 10 minutes and cultured in MYPGP agar. The samples were ccharacterized by macroscopic and microscopic features. Than DNA isolations of the isolates were made, 16S rRNA was identified molecularly according to gene sequence analysis and phylogenetic tree. In molecular diagnosis, E2-1A and E21-1A strains (100% and 96.5%, respectively) were found to be similar to Paenibacillus cookii strain L5 and E176-1B strain 100% Paenibacillus lautus strain FJAT-45556. Motility is crucial as it enables bacteria to seek out and colonize suitable environments. Motile bacteria face rapid environmental changes and thus has to respond to those changes much faster. Moreover, also facilitates the spread of other non-motile microbes and fungi that bind to motily colonies in their habitats. Many Paenibacillus species stimulate plant growth; for example, by aiding in nutrient intake through nitrogen fixation and by producing cytokines, peptide antibiotics and volatile substances that elicit a defense response. It is thought that the isolated and characterized strains in our study are visually quite striking strains and their use in bioremediation by determining their properties promoting plant growth will be beneficial.

Keyword: Swarming bacteria, Paenibacillus lautus, P. cookie, honeybee