Akademik Veri Yönetim Sistemi
XXXI. Balkan Klinik Laboratuvar Federasyonu ve 35. Ulusal Biyokimya Kongresi, Antalya, Türkiye, 28 Ekim - 01 Kasım 2024, ss.91, (Özet Bildiri)
Department of Medical Biocehmistry, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Türkiye Objectives: Biochemical tests, such as total protein and lactate dehydrogenase (LDH), are crucial in distinguishing between transudate and exudate in body fluids, alongside clinical history and physical examination. We aim to assess the impact of reporting total protein results that fall below the measurement limits on the classification of transudate and exudate. Methods: 920 pleural and 164 peritoneal fluid sample results between 2022 and 2024 were evaluated. Serum total protein, body fluid protein, serum LDH, and body fluid LDH results were obtained. The limit of quantification (LOQ) for the total protein reagent was 30 g/L, while the limit of detection (LOD) was 0.77 g/L. Light’s criteria was calculated based on both the LOQ and LOD values, and the agreement between the results was evaluated using kappa analysis. Results: In pleural and peritoneal fluids, transudate/ exudate counts were 111/809 and 37/127, respectively, when total protein values were reported according to the LOQ. There were 173/747 and 79/85 transudate/ exudate counts in pleural and peritoneal fluids, respectively, when values above the LOD were considered. The agreements of the results were found to be good (kappa=0.744) for pleural fluids and moderate (kappa=0.477) for peritoneal fluids. Conclusions: The total protein results of body fluids significantly affects clinical decisions. Therefore, deciding whether to use LOQ or LOD in reporting total protein analysis is crucial. Reagents with lower LOQ values are needed for the measurement of total protein in body fluids. Keywords: Body fluid, detection capability, limit of quantification