Characterization of a nucleopolyhedrovirus variant of the gypsy moth, Lymantria dispar (Lepidoptera: Lymantriidae) in Turkey


Bayramoglu Z. , Gencer D., MURATOĞLU H., Efe D., NALÇACIOĞLU R., DEMİRBAĞ Z., ...Daha Fazla

INTERNATIONAL JOURNAL OF PEST MANAGEMENT, cilt.64, sa.2, ss.119-127, 2018 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 64 Konu: 2
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1080/09670874.2017.1344789
  • Dergi Adı: INTERNATIONAL JOURNAL OF PEST MANAGEMENT
  • Sayfa Sayıları: ss.119-127

Özet

The gypsy moth (Lymantria dispar L., Lepidoptera: Lymantriidae) is one of the most serious pest of various forestal, food and industrial crops worldwide. We have characterized a new Lymantria dispar nucleopolyhedrovirus (LdMNPV-T4) variant, which was isolated from dead L. dispar larvae in Turkey. Scanning electron microscope observations showed that the polyhedral occlusion bodies (OBs) of the LdMNPV-T4 were irregularly shaped. Transmission electron microscopy revealed that OBs of LdMNPV-T4 were occupied with several virions in which multiple nucleocapsids packaged by viral envelope. Restriction analysis of the LdMNPV-T4 DNA purified from the viral inclusion bodies yielded BamHI, BglII, EcoRI and HindIII fragments. The mean size estimated for the complete LdMNPV-T4 genome was calculated to be 163.3kb. Phylogenetic analysis of amplified polh, lef-8 and lef-9 sequences showed its relation to the other NPVs from Lymantria species. Mortality values of the LdMNPV-T4 at four different concentrations against third instar larvae of L. dispar ranged from 45% to 88%. These results suggest that LdMNPV-T4 isolated from Turkey is a promising microbial control agent to be utilized for the biological control of L. dispar.