Evaluation of the Effectiveness of Entomopathogens for the Control of Colorado Potato Beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)


Eski A., Bayramoğlu Z. , Sönmez E., Biryol S., Demir İ.

Journal Of Agricultural Science And Technology, vol.24, no.2, pp.393-405, 2022 (Journal Indexed in SCI Expanded)

  • Publication Type: Article / Article
  • Volume: 24 Issue: 2
  • Publication Date: 2022
  • Title of Journal : Journal Of Agricultural Science And Technology
  • Page Numbers: pp.393-405

Abstract

Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

damages potato, tomato, and eggplant, and is one of the most serious agricultural pests all

over the world. Due to its resistance against chemical insecticides and some biopesticides,

new biocontrol agents compatible with different ecological conditions are needed urgently

for the integrated pest management programs of this pest. For this purpose, we studied

the insecticidal effects of thirteen indigenous microbial isolates including

entomopathogenic bacteria, fungi, and nematodes from our culture collection against L.

decemlineata with screening and dose-response tests under laboratory conditions. Bacillus

thuringiensis strain Xd3 caused 83% and 73% mortality against larvae and adults of the

pest at 109 CFU mL-1 concentration within 10 days, respectively. While fungal isolate

Metarhizium anisopliae Gg-12 yielded 98% mortality with 107 conidia mL-1 concentration

on larvae at 15 days, mortality provided by Gg-12 on adults reached 100% at the same

concentration and period. Steinernema websteri AS1 was determined as the most effective

entomopathogenic nematode with 92% mortality within seven days on larvae. Based on

probit analysis, the LC50 values of B. thuringiensis Xd3 against larvae and adults were

calculated as, respectively, 1.73×106 and 1.69×107 CFU mL-1, and that of M. anisopliae

Gg12 were 1.18×104 and 6.2×103 conidia mL-1, and that of S. websteri AS1 was 117 IJs mL-

1. Considering these results, the biopesticides developed from these isolates can be used

safely and successfully in the pest management control programs of Colorado potato

beetle.