Molecular characterization of novel resynthesized rapeseed (Brassica napus) lines and analysis of their genetic diversity in comparison with spring rapeseed cultivars


Seyis F., Snowdon R., Lühs W., Friedt W.

PLANT BREEDING, vol.122, no.6, pp.473-478, 2003 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 122 Issue: 6
  • Publication Date: 2003
  • Doi Number: 10.1111/j.1439-0523.2003.00859.x
  • Journal Name: PLANT BREEDING
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.473-478
  • Keywords: Brassica napus, Brassica oleracea, Brassica rapa, AFLP, genetic diversity, rapeseed, resynthesized, RAPD MARKERS, RFLP, POLYMORPHISMS, AFLP
  • Recep Tayyip Erdoğan University Affiliated: No

Abstract

Resynthesized (RS) rapeseed generated from interspecific hybridization between suitable forms of Brassica rapa L. (syn. campestris; genome AA, 2n = 20) and B. oleracea L. (CC, 2n = 18) represents a potentially important resource to expand genetic diversity in the narrow gene pool of oilseed rape (B. napus L., AACC, 2n = 38). In this study 165 RS rapeseed lines originating from crosses between an Indian Yellow Sarson (B. rapa ssp. trilocularis) and five different cauliflower (B. oleracea convar. botrytis) cultivars were studied using amplified fragment length polymorphism (AFLP) markers and their genetic diversity was compared in relationship to an assortment of 40 diverse spring oilseed and fodder rape varieties. Using three AFLP primer combinations, a total of 467 polymorphic bands were scored. Cluster analysis allowed differentiation among the different RS lines, which, as expected, were genetically highly divergent from the cultivars. The genetic diversity of the material is discussed in relation to its morphological variability with a view to the implementation of RS lines in oilseed rape breeding.