Akademik Veri Yönetim Sistemi
The International Congress of Health Disciplines, Bilecik, Türkiye, 4 - 05 Şubat 2026, ss.108-122, (Tam Metin Bildiri)
Abstract
Small cell lung cancer (SCLC) represents one of the most aggressive subtypes of lung
malignancies, characterized by rapid proliferation and poor therapeutic outcomes. To facilitate
the study of SCLC biology and to evaluate the cytotoxic potential of novel agents, the H209
cell line—derived from human SCLC tissue—has been extensively utilized as an in vitro model
that closely mirrors the proliferative behavior of this cancer type.
Syringic acid (SA) is a naturally occurring compound known for its diverse biological
activities. This study aimed to investigate the cytotoxic and morphological effects of SA on the
H209 small-cell lung carcinoma cell line. Cells were treated with increasing concentrations of
SA (100, 200, 400, 800, and 1200 µg/mL) for 48 hours. Cytotoxicity and cell viability were
assessed using the MTT and Trypan Blue exclusion assays, while fluorescence microscopy with
Hoechst 33342 and Propidium Iodide (PI) staining was employed to distinguish viable and non-
viable cells. Morphological changes were examined under an inverted light microscope to
evaluate dose-dependent alterations in cell structure and adherence. The MTT results revealed
a concentration-dependent decrease in cell viability, with approximately 50% cytotoxicity
observed at 1200 µg/mL (p < 0.001). Lower concentrations (100–800 µg/mL) exhibited
gradually reduced inhibitory effects. Trypan Blue and fluorescence staining analyses confirmed
the loss of membrane integrity and a decrease in viable cell numbers at higher concentrations.
Morphological observations supported these findings, showing reduced cell density and
structural disruption in treated groups compared to controls. Collectively, these results indicate
that syringic acid exerts a dose-dependent cytotoxic effect on H209 cells, demonstrating
potential as a bioactive compound for further in vitro cellular response studies.
Keywords: Syringic acid, small cell lung cancer, H209 cells, cytotoxicity, cell viability