Identification and pathogenic characterization of Fusarium species isolated from root rot of tomatoes in Rize


ALPAY KARAOĞLU Ş. , Kasap Y., Veyisoğlu G., Pınarbaş M., Bozdeveci A., Tarakçi C., ...More

1St International Eurasia Mycology Congress, Manisa, Turkey, 3 - 05 July 2017, vol.P-45, pp.171-0

  • Publication Type: Conference Paper / Full Text
  • Volume: P-45
  • City: Manisa
  • Country: Turkey
  • Page Numbers: pp.171-0

Abstract

In this study, it was aimed to isolate and identify the microfungi that causing the plant disease from tomato (root, stem and fruit) and soil samples. All Fusarium strains collected during this study were used to detect their pathogenicity against germination of tomato seeds. According to the soil samples dilution method, plant samples were homogenized after surface sterilization and planted on DRBC medium. All the isolates were grown on PDA at room temperature (26 ± 2°C). Species were differentiated according to the morphology of the macroconidia, microconidia and their arrangement in chains or false heads, the type of conidiophore, and the presence or absence of chlamydospores. Using ITS region sequencing, all collected fungi were identified at the species level. All Fusarium strains collected during this study were used to detect their pathogenicity and the vigor index against to germination of tomato seeds.   A total of 25 specimens were collected from soil and tomatos (root, stem and fruit) from which root rot disease was observed. A total of 14 Fusarium spp. were detected from PDA agar media in according to the morphological characteristics. The species were identified as F. oxysporum, F. solani, F. sambucinum and Fusarium spp. among the species, F. sambucinum was the most frequently detected. In the seed assay, germination success was determined to decrease in the seeds infected with Fusarium spp. according to the control groups. There was statistically significant difference between control and Fusarium spp. Yp4c, Yp13b and Yp18b strains compared to Vigor index (p <0,05). Fusarium spp Yp4c, Yp13b and Yp18b strains were identified as the most pathogenic strains from tomato germination. This is the first report on the identification of major pathogenic fungi isolated from tomato root rot disease in Rize. Keywords: Fusarium oxysporum, tomato, soil, Rize