Akademik Veri Yönetim Sistemi
CRISPR-Cas9 as a Revolutionary Gene Editing Tool: Genome Engineering, Advances and Applications in Agriculture, CRC Press, ss.19-33, 2026
The accurate genome editing capabilities of CRISPR have led to significant progress in biotechnology and plant biology. This chapter represents an extensive overview of the molecular processes behind CRISPR/Cas-mediated genome editing and its uses in plant development. The crucial fundamentals of the CRISPR/Cas system, involving the Cas nuclease and guide RNA, have been examined to understand how they recognize, cleave, and target DNA. Moreover, the present advancements in CRISPR/Cas-based techniques for precise manipulation of genes, control of gene expression, and study of gene functions in plants. We emphasize significant accomplishments in crop enhancement, such as increasing agronomic characteristics, increasing stress resistance, and regulating metabolic pathways. Furthermore, the opportunities and obstacles posed by CRISPR/Cas technology are examined, involving off-target impacts, legal issues, and ethical issues. This chapter concludes by outlining the prospects and exciting possibilities for CRISPR/Cas research proceeding forward, which incorporates the creation of innovative genome editing tools, their integration with omics techniques, and possible uses for food security and agricultural sustainability. In general, this chapter offers valuable knowledge on the molecular processes and recent developments in CRISPR/Cas technology that are influencing crop enhancement methods and plant development.