The nephroprotective effect of amifostine in a cecal ligation-induced sepsis model in terms of oxidative stress and inflammation

Batcik Ş., Tumkaya L., Mercantepe T., Atak M., Topcu A., Uydu H. A., ...More

EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, vol.26, no.24, pp.9144-9156, 2022 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 26 Issue: 24
  • Publication Date: 2022
  • Journal Name: EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, MEDLINE, Directory of Open Access Journals
  • Page Numbers: pp.9144-9156
  • Keywords: Key Amifostine, Inflammation, Kidney, Oxidative stress, Sepsis
  • Recep Tayyip Erdoğan University Affiliated: Yes

Abstract

-OBJECTIVE: Sepsis is responsi-ble for more than 5 million deaths worldwide ev-ery year. The purpose of this study was to use amifostine to reduce acute kidney injury devel-oping as a result of sepsis.MATERIALS AND METHODS: Thirty Sprague Dawley rats were divided into three equal groups - a healthy control group (Group 1), cecal liga-tion and puncture group (CLP, Group 2), and a CLP + amifostine (AMF) group receiving a total of 200 mg/kg AMF intraperitoneally (i.p.) 15 min before sepsis induction (Group 3).RESULTS: Total thiol levels decreased while malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-alpha), nuclear factor kappa B (NF-Kappa B/p65), and interleukin (IL)-1 beta, and IL-6 levels increased in the CLP group. We also observed degeneration in re -nal corpuscles, necrotic tubules, polymorphonu-clear leukocyte inflammation, and vascular con- gestion. In the amifostine group, total thiol levels in tissue increased, while MDA, TNF-alpha, NF-kB/ p65, IL-1 beta, and IL-6 levels, necrotic renal tubules, and inflammation decreased.CONCLUSIONS: Amifostine prevented sep-sis-related acute kidney injury by reducing in-flammation and oxidative stress.