Effects of melatonin and omega-3 on number of granular cells of the rat dentate gyrus exposed to electromagnetic field (EMF)

2nd Asian and African Stereology Congress, Dubai, Birleşik Arap Emirlikleri, 2 - 05 Aralık 2015, (Özet Bildiri)

Nowadays; free electromagnetic field (EMF) is quite high in outdoor with the

discovery of novel devices. This sources leads proliferation of free radicals in

intracellular area by means of reactive oksigen species (ROS). Melatoin and

omega-3 (ω-3) are two well-known antioxidative agents in recent literature. The

aim of our study was to investigate the celular effects of 900 MHz EMF exposure

and neuroporective efficiency of Mel and w-3 on damaged granular cells in

dentate gyrus of adult rats. For this purpose; selected animals were randomly

divided into four equal groups (n=6). The Cont group that was not exposed to any

procedure; the EMF group, exposed to 900 MHz electromagnetic field for 1h/day

group; the EMF+Mel group, 50 mg/kg/day Mel (Sigma-Aldrich Comp., St Louis,

MO, USA) injected intraperitoneally (i. p.) group during 900 MHz

electromagnetic field exposure time (Jahnke et al., 1999) and the EMF+ω3 group,

animals fed with standard feed added Ω3 substances (Sigma Chemical Comp., St

Louis, MO, USA) %10 of standard feed. Granular cells were estimated using the

optical fractionator, which is one of unbiased stereological techniques, by

stereoinvestigator analysing system. GSH, CAT and SOD enzyme activities have

determined at respectively 410 nm, 540 nm, and 450nm. When the granular cell

numbers of the groups were estimated, although a highly significant decrease in

EMF group compared to other study groups (p<0.01), in terms of granular cell

numbers for each group, there was no significantly difference between EMF+Mel

and Cont, EMF+ω3 and Cont, EMF and Cont (p> 0.05). Similarly, there was no

significantly difference between EMF+ω3 and EMF+Mel groups. Also, the

activities of CAT, total GSH, and SOD in serum among EMF, Cont, EMF+Mel,

EMF+ω3 (A-C) was shown by using spectrophotometer. There was no

significantly difference between Cont and EMF+ω3 in terms of CAT and total

GSH activities. Additionally, Serum SOD activity did not show statistically

significant differences among the Cont, EMF+Mel, and EMF+ω3 (p> 0.05). There

was highly significant increase in serum enzyme activities of EMF compared to

other study groups (p <0.01). When examined the serum CAT and total GSH

activities of EMF+Mel, there was highly significant increase in comparison with

Cont and EMF+ω3 (p<0.01). In conclusion, EMF reduced number of granuler

cells in dentate gyrus. Using Mel and ω-3 have neuroprotective effect if used after

EMF exposure.