Anti-inflammatory and antioxidant effects of infliximab in a rat model of intestinal ischemia/reperfusion injury


PERGEL A. , Kanter M., Yucel A. F. , AYDIN I. , Erboga M., Guzel A.

TOXICOLOGY AND INDUSTRIAL HEALTH, vol.28, no.10, pp.923-932, 2012 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 28 Issue: 10
  • Publication Date: 2012
  • Doi Number: 10.1177/0748233711427056
  • Title of Journal : TOXICOLOGY AND INDUSTRIAL HEALTH
  • Page Numbers: pp.923-932
  • Keywords: Intestinal ischemia/reperfusion (I/R), oxidative stress and inflammation, proliferation and apoptosis, infliximab, rat, ISCHEMIA-REPERFUSION INJURY, FIBROBLAST-GROWTH-FACTOR, CELL-DEATH, NEUTROPHIL INFILTRATION, LIPID-PEROXIDATION, OXYGEN RADICALS, ALPHA THERAPY, APOPTOSIS, PRETREATMENT, MECHANISM

Abstract

The aim of this study was to investigate the possible protective effects of infliximab on oxidative stress, cell proliferation and apoptosis in the rat intestinal mucosa after ischemia/reperfusion (I/R). A total of 30 male Wistar albino rats were divided into three groups: sham, I/R and I/R+ infliximab; each group comprised 10 animals. Sham group animals underwent laparotomy without I/R injury. I/R groups after undergoing laparotomy, 1 hour of superior mesenteric artery ligation occurred, which was followed by 1 hour of reperfusion. In the infliximab group, 3 days before I/R, infliximab (3 mg/kg) was administered intravenously. All animals were killed at the end of reperfusion and intestinal tissues samples were obtained for biochemical and histopathological investigation in all groups. To date, no biochemical and histopathological changes have been reported regarding intestinal I/R injury in rats due to infliximab treatment. Infliximab treatment significantly decreased the elevated tissue malondialdehyde levels and increased reduced superoxide dismutase and glutathione peroxidase enzyme activities in intestinal tissues samples. I/R caused severe histopathological injury including mucosal erosions, inflammatory cell infiltration, necrosis, hemorrhage, and villous congestion. Infliximab treatment significantly attenuated the severity of intestinal I/R injury, inhibiting I/R-induced apoptosis, and cell proliferation. Because of its anti-inflammatory and antioxidant effects, infliximab pretreatment may have protective effects on the experimental intestinal I/R model of rats.