Investigation of Notch Pathway Responsive Gene Expressions in Colon Cancer

Boz Er A. B.

Rize Medical Journal, cilt.1, sa.1, ss.1-7, 2025 (Hakemli Dergi)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1 Sayı: 1
  • Basım Tarihi: 2025
  • Dergi Adı: Rize Medical Journal
  • Derginin Tarandığı İndeksler: Other Indexes
  • Sayfa Sayıları: ss.1-7
  • Recep Tayyip Erdoğan Üniversitesi Adresli: Evet

Özet

Aim: Colorectal cancer is a leading cause of cancer-related

mortality, due to therapy resistance and metastasis. This

study explored the role of Notch signaling in colorectal

cancer by comparing the expression of pathway-associated

genes between healthy CCD841-con cells and HCT116

colorectal cancer cells.

Findings: Semi-quantitative PCR analysis revealed

significant upregulation of GATA3 (4-fold), HES1 (5-

fold), and HEY1 (3-fold) in HCT116 cells, while PTCRA

expression decreased 4-fold (p<0.005, normalized to

GAPDH). These results suggest that Notch signaling

contributes to tumor progression in colorectal cancer. The

elevated expression of HES1 and HEY1 may be linked to

epithelial-mesenchymal transition and cancer stem cell-

like traits.

Conclusion: Overall, this study highlights the potential of

targeting the Notch pathway in colorectal cancer therapy,

offering insights into its role in tumor development and

resistance mechanisms.