Pseudomonas species isolated from rainbow trout (Oncorhynchus mykiss) in the Eastern Black Sea Region of Turkey were identified on the basis of three different genes (16S rRNA, rpoD and rpoB). Different Pseudomonas strains were analyzed using biochemical tests and PCR asssays designed to for Pseudomonas species. The rpoD gene was show to be more discriminatory than the 16S rRNA and rpoB genes. Phylogenetic analyses discerned two main groups as Pseudomonas fluorescens and Pseudomonas putida. The Pseudomonas strains used in this study were mainly identified as Pseudomonas fluorescens or Pseudomonas putida although other Pseudomonas spp. were characterised.