Akademik Veri Yönetim Sistemi
26. Ulusal Elektron Mikroskopi Kongresi, Eskişehir, Türkiye, 20 - 23 Eylül 2023, ss.55, (Özet Bildiri)
Signals sent from injured axons to the cell body cause dramatic changes in sensory neurons in the dorsal
root ganglion, where the cell bodies of damaged axons are located. The weakening of the connection
between the distal and proximal stumps of the damaged nerve causes severe plasticity in the ganglion
cells and macrophage-like behaviour of the satellite cells (such as autophagosome formation). This reveals
the flexibility of satellite cells in the tissue. Sensory neurons and satellite cells respond synchronously to
the injury and communicate well thanks to their wide connection surface. The changing cellular
environment in the ganglion and the balance of various inflammatory agents (IL-1 , IL-6, IL-12 TNF- , TNF-
) give rise to various changes that lead to cell death, new cell formation or differentiation of the satellite
cells. In addition to showing apoptotic and necrotic cell morphology, histomorphometric changes such as
the formation of autophagic vacuoles, cytosolic deterioration, chromatolysis, etc., are commonly seen in
cells after injury.
In nerve regeneration studies, stereological and electron microscopic evaluation of peripheral nerve
tissues and ganglia is the gold standard for evaluating regenerative therapy's success, neuropathic pain
models and treatment methods, and measuring the results of pharmacological and bioengineering
applications. The reproducible, unbiased, and observer-independent feature of stereology makes the
technique powerful in quantitatively handling 3D structures based on their 2D dimensions. Using the
disector and fractionator principles in stereological methods provides unbiased data in evaluating
parameters such as length, area, number, and volume. The basic principle of these techniques is to give
an equal chance of sampling the structure to be assessed (e.g., cell body or nerve fibre).
In this presentation, I will give an overview of the histomorphometric and stereological evaluation of
sensory neurons and satellite cells after sciatic nerve injuries.