Evaluation of Antioxidant and Antimicrobial Effects on Extracts of (Hypericum perforetum L.) Obtained by Different Methods


KARA B., KOBYA O., ÇAĞLAK E., KARSLI B. , ÖĞRETMEN Ö. Y.

HASAT International Agriculture and Forest Congress, Ankara, Turkey, 21 - 23 June 2019, pp.112-113

  • Publication Type: Conference Paper / Summary Text
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.112-113

Abstract

The therapeutic properties of medicinal plants have been used since ancient times. One of these plants, the hypericum perforatum L. is used in the treatment of various diseases such as cancer, diabetes, depression, stomach ulcers, chronic rheumatism, jaundice, dysentery, throat infections, and wound healing.In the studies which were conducted, it has been stated that the hypericum perforatum has antitumor, antiviral, antidepressant, antibacterial, antioxidant, and antimicrobial effects. Antioxidant and antimicrobial effects of this plant are mainly used as burn and wound healing. In this study, antioxidant and antimicrobial effects of the hypericum perforatum extracts obtained from dried flowers and branches by natural methods were evaluated. The hypericum perforatum in small pieces was extracted with ethanol (25 ° C), warm water (25 ° C) and hot water (75 ° C) at different concentrations (25g / L, 50g / L) for 24 hours. DPPH (1,1-diphenyl-2-picryl-hydrylase) method was used to determine the antioxidant activity of hypericum perforatum. When antioxidant data were compared, it was determined that the plants prepared with warm water showed better antioxidant activity than the groups prepared with hot water and ethanol. The antimicrobial activities of the samples were determined by disk diffusion method against Escherichia coli, Staphylococcus aureus, Salmonella enterica, and Listeria monocytogenes.