XVII SOLANACEAE2022 International Conference on the Plant Family of Solanaceae, Thessaloniki, Greece, 2 - 05 November 2022, pp.43-44
Among the Solanacea crops pepper – Capsicum spp – is the only one that can make one
either smile or cry owing to presence of specific metabolites which pepper fruits
accumulate. Diverse and recognizable flavor of bell pepper or chilli pepper is determined
by specific volatile compounds, pungency, caused by capsaicinoids, is essencial in some World’s cuisines, fruit color is determined by carotenoids and flavonoids, nutritional
quality – by vitamins and antioxidants. World’s gene banks possess a great genetic and
phenotypic diversity of pepper that could be used to diversify the cultivated pepper
germplasm. To study how the genetic diversity of the most cultivated pepper species C.
annuum translates into metabolic diversity ripe fruits of a core collection of 450
accessions developed in the EU Horizon 2020 G2P-SOL project (http://www.g2psol.eu/) were profiled using different metabolomics platforms for volatile aroma
compounds using GC-MS and for non-volatile secondary metabolites using LC-MS.
Wide qualitative and quantitate metabolic variation was observed. Volatile esters,
alkanes, terpenes and oxylipin volatiles determined the variation in the composition of
aroma compounds. Flavonoids and capsaicinoids had the major contribution to the
variation in semi-polar secondary metabolite composition. Compounds from the abovementioned groups revealed highly similar quantitative patterns across the core collection
suggesting a coordinate regulation of compounds that belong to the same biosynthetic
origin. GWAS was performed using SNP data obtained using genotyping-by-sequencing
and revealed multiple associations of the metabolic variation with genomic loci
harboring known and novel candidate genes.