RAPD-based study of genetic variation and relationships among wild fig genotypes in Turkey

Akbulut M., ERCİŞLİ S., Karlidag H.

GENETICS AND MOLECULAR RESEARCH, vol.8, no.3, pp.1109-1115, 2009 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 8 Issue: 3
  • Publication Date: 2009
  • Doi Number: 10.4238/vol8-3gmr634
  • Journal Name: GENETICS AND MOLECULAR RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1109-1115
  • Keywords: Wild fig, Ficus, Random amplified polymorphic DNA, Genetic diversity, CARICA, RELATEDNESS
  • Recep Tayyip Erdoğan University Affiliated: No

Abstract

The fig tree (Ficus carica L.) is of significant socioeconomic importance in Turkey, with 25% of the world's fig production. Genetic variation and relationships among 14 wild-grown figs sampled from Coruh Valley in Turkey were characterized by random amplified polymorphic DNA (RAPD). Ninety-eight DNA fragments were scored after amplification of DNA samples with 13 random primers; 70% of the scored bands were polymorphic. Genetic distances between the fig genotypes ranged from 0.21 to 0.62. Genotypes 08-ART-02 and 08-ART-06 were found to be the most closely related, whereas 08-ART-09 and 08-ART-10 were the most distant. The 14 wild-grown genotypes were grouped into six main clusters and one outgroup. We conclude that RAPD analysis is efficient for genotyping wild-grown fig genotypes.