Microorganisms that Reproduce in Wound Cultures in Rize Region and Their Antimicrobial Susceptibility


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Bahçeci İ.

Middle Black Sea Journal of Health Science , cilt.6, sa.3, ss.358-368, 2020 (Hakemli Üniversite Dergisi)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 6 Konu: 3
  • Basım Tarihi: 2020
  • Doi Numarası: 10.19127/mbsjohs.823556
  • Dergi Adı: Middle Black Sea Journal of Health Science
  • Sayfa Sayıları: ss.358-368

Özet

Abstract Objective: Among nosocomial infections, wound infections are one of the important factors causing mortality and morbidity after urinary tract infections. In this study, it was aimed to guide the empirical treatment and to contribute to epidemiological data by determining the microorganism and antimicrobial susceptibility which have been isolated from cultures of wounds and abscesses, which came from outpatient clinics, services and intensive care units, that grow in wound site and abscess cultures that comes from outpatient clinics, services and intensive care units. Methods: This study was conducted on the basis of cultures in which at least one microorganism grew in the wound site and abscess samples from the polyclinic, service and intensive care units during routine application at the Recep Tayyip Erdoğan University Training and Research Hospital Microbiology Laboratory, between January 2011 and December 2016. Samples were taken with two sterile swabs in the form of superficial swabs or deep aspiration and delivered to the laboratory with transport medium as soon as possible. Gram staining preparation was prepared and inoculated on 5% sheep blood agar, eosinmethylene-blue agar, chocolate agar and sabouraud dextrose agar. The gram-stained preparation was evaluated by Q scoring. Evaluation of the culture and antibiogram susceptibility were made according to the Clinical and Laboratory Standards Institute (CLSI) criteria. Results: A total of 2202 samples were received, and reproduction was observed in 930 samples. Among the 793 wound and 137 abscess samples, the most common microorganism was Staphylococcus aureus (224 cultures) and the second was Escherichia coli (135 cultures). Conclusion: Surgery clinics, especially orthopedics, provided the most common wound infections, from which S. aureus was the most isolated microorganism. The fact that bacterial, most notably S. aureus, propagation occurred from surgery samples indicates that surgical site infections are generally caused by endogenous flora. It has, thus, become apparent in our study that patients and hospital staff should pay more attention to hygiene, especially hand washing. Due to the changing of the distribution and resistance patterns of microorganisms that are frequently seen in hospitals at certain time intervals, their antibiotic susceptibility will be a guide in the rational use of antibiotics. Thus, specific treatment will contribute to the saving on the cost and reduce mortality.