Molecular Identification of Astacus leptodactylus and Austropotamobius torrentium Using mtDNA-RFLP Method

Kalayci G., AKHAN S.

TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES, cilt.16, sa.4, ss.789-795, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 16 Sayı: 4
  • Basım Tarihi: 2016
  • Doi Numarası: 10.4194/1303-2712-v16_4_05
  • Dergi Adı: TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.789-795
  • Anahtar Kelimeler: crayfish, species identification, mtDNA, COI, WHITE-CLAWED CRAYFISH, FRESH-WATER CRAYFISH, PCR-RFLP, SPECIES IDENTIFICATION, POPULATION-STRUCTURE, MITOCHONDRIAL-DNA, PALLIPES, DIVERSITY
  • Recep Tayyip Erdoğan Üniversitesi Adresli: Evet

Özet

mtDNA-RFLP, a technique commonly used for molecular identification of species, was used to distinguish between morphologically similar crayfish species Austropotamobius torrentium and Astacus leptodactylus in this study. The mitochondrial COI gene was amplified and digested using 15 restriction endonucleases to establish a restriction map. The COI gene sequence contained recognition sites for three of the restriction endonucleases (SspI, KpnI, and RsaI) and produced two fragments and three genotypes for Austropotamobius torrentium. However, the Astacus leptodactylus COI gene did not have restriction sites for these three enzymes. Thus, a mtDNA-RFLP using these three endonucleases can be used to successfully distinguish between these two species of crayfish.