Biosorption of Heavy Metals by Anoxybacillus gonensis Immobilized on Diaion HP-2MG

DURAN C., Bulut V. N., GÜNDOĞDU A., SOYLAK M., BELDÜZ A. O., Beris F. Ş.

SEPARATION SCIENCE AND TECHNOLOGY, vol.44, no.2, pp.335-358, 2009 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 2
  • Publication Date: 2009
  • Doi Number: 10.1080/01496390802437131
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.335-358
  • Keywords: Anoxybacillus gonensis, biosorption, diaion HP-2MG, flame atomic absorption spectrometry, preconcentration, trace metals, SOLID-PHASE EXTRACTION, ATOMIC-ABSORPTION SPECTROPHOTOMETRY, POTASSIUM-PERMANGANATE, AMBERLITE XAD-4, TABLE SALTS, PRECONCENTRATION, REMOVAL, CADMIUM, IONS, COBALT(II)
  • Recep Tayyip Erdoğan University Affiliated: No


The determination of trace metal ions usually requires previous separation and preconcentration stages in order to cope with low levels and to remove the interfering components. Nowadays emphasis is given to the utilization of microorganisms because of their great ability to absorb metal ions from aqueous solution. In this paper, for this, Zn2+, Fe3+, Cu2+, Cd2+, Ni2+, Co2+, and Pb2+ ions at trace levels have been separated and preconcentrated on a column containing a bacterium, Anoxybacillus gonensis immobilized on Diaion HP-2MG as a new biosorption system prior to their atomic absorption spectrometric determinations. The effects of some analytical parameters were investigated. Optimum pH values were found to be 6 for Zn, Fe, Cu and Pb, 8 for Cd, Ni, and Co. Recoveries of Zn2+, Fe3+, Cu2+, Cd2+, Ni2+, Co2+, and Pb2+ were 953, 986, 962, 982, 972, 954 and 953 at 95% confidence level, respectively. No significant matrix interferences on the quantitative recoveries of the analyte ions were observed. Preconcentration factors of the anlayte ions were calculated as 50 for Zn, Cd and Pb, and 75 for Fe, Cu, Ni, and Co. The limits of detection for the analyte ions were in the range 0.2-1.3 g L-1. The procedure was validated by spike addition and analysis of standard reference materials.