JOURNAL OF APICULTURAL RESEARCH, 2026 (SCI-Expanded, Scopus)
Galleria mellonella (Greater wax moth) is one of the most important pests that cause economic losses in beekeeping. The larvae of the greater wax moth cause serious damage to combs, especially those stored in dark, hot and poorly ventilated rooms. Despite various applications, the damage caused by the pest in beekeeping continues. It is therefore extremely important to develop new and effective means and methods to control the pests. The aim of this study was to determine the efficacy of baculovirus strains from our baculovirus culture collection on G. mellonella larvae and to develop a prototype viral biopesticide that can be used in the control of the pest. Initially, baculovirus isolates and strains common in our country, Autographa californica multiple nucleopolyhedrovirus (AcMNPV), Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV & times; 4), Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV & times; 2) and Hyphantria cunea granulovirus (HycuGV) were tested on G. mellonella larvae in a screening test. These tests showed that AcMNPV had the highest effect on the pest with a mortality of 30% at a concentration of 1 & times; 10(7) PIBs/ml for 14 days. In the dose-response test, 1 & times; 10(9) PIB/ml concentration of AcMNPV produced 59% insecticidal effect 14 days after infection, and the LC50 value of AcMNPV was calculated as 5.6 & times; 10(7) PIBs/ml (1 & times; 107-108, X-2 = 0.828). The liquid viral prototype biopesticide developed from AcMNPV yielded a 64% insectisidal effect with 1 & times; 10(9) PIB/ml on G. mellonella larvae. The same biopesticide did not show any effect on honey bees.