INVESTIGATION OF PROPAGATION POSSIBILITIES OF Thymus praecox (Anzer Thyme) WITH TISSUE CULTURE


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Yurteri E. , Sav N., Küplemez H. , Seyis F.

Vth INTERNATIONAL EURASIAN AGRICULTURE AND NATURAL SCIENCES CONGRESS, Konya, Turkey, 23 December 2021, vol.1, no.1, pp.24-28

  • Publication Type: Conference Paper / Full Text
  • Volume: 1
  • City: Konya
  • Country: Turkey
  • Page Numbers: pp.24-28

Abstract

Abstract
Anzer Thyme (Thymus praecox Opiz subsp. caucasicus var. caucasicus) is named after the Anzer plateau in Rize, Turkey, which is located in the East Black Sea region. It is a member of the genus Thymus (Lamiaceae family), which has 41 species in Turkey, 24 of which are endemic. Anzer tea is made from fresh and dried Thymus praecox Opiz subsp. caucasicus var. caucasicus leaves. It is also used as a traditional medicine to cure stomach aches, headaches, influenza, cough, and excessive cholesterol. In this study, plant propagation of Thymus praecox Opiz subsp. caucasicus var. caucasicus (Anzer Thyme) in tissue culture conditions was investigated.  In order to induce plant regeneration 1 mg/L Kinetin and 1 mg/L Kinetin + 0.1 mg/L NAA were tested on leaf, stem and root explants. MS medium without any plant growth regulators was preferred as control group. All explants were cultured in two different incubation conditions respectively as; 16 hours light and 8 hours dark conditions at 26°C, 3000 lux light source in growth chamber and dark condition at 26°C. According to the results, the highest plant regeneration rates were found in 1 mg/L Kinetin + 0.1 mg/L NAA medium with root, stem and leaf explants respectively as 51.3%, 40% and 27.5%. The lowest plant regeneration rate was found as 5.6% in leaf explants in control medium. As a result, only 1 mg/L Kinetin treatments were efficient in inducing plant regeneration (35.7% in stem, 25.7% in root and 18.8% in leaf explants) but when combined with 0.1 mg/L NAA demonstrated higher regeneration rates.