Phylogenetic analysis of tea clones (Camellia sinensis) using RAPD markers


Beris F. Ş. , Sandalli C., CANAKCI S., DEMIRBAG Z., BELDUZ A.

BIOLOGIA, cilt.60, ss.457-461, 2005 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 60 Konu: 4
  • Basım Tarihi: 2005
  • Dergi Adı: BIOLOGIA
  • Sayfa Sayıları: ss.457-461

Özet

The random amplified polymorphic DNA (RAPD) technique offers a useful tool to detect DNA polymorphisms. It can be used to distinguish different clones and cultivars. These markers also represent an efficient and inexpensive way to generate molecular data and thus, have been used successfully in various taxonomic and phylogenetic studies. We used this technique to determine the genetic similarity of 6 tea clones [Camellia sinensis (L.) O. KUNTZE] grown in Turkey (but one clone belongs to Georgia) by using 10 decamer primers. The genetic relationship among clones was estimated based on similarity index and cluster analysis. The lowest similarity (0.530) obtained was found between "Ciftekavak" and "Ardesen", the highest similarity (0.730) obtained was between "Ardesen" and "Kolhida". Utilising cluster analysis, 2 clones were classified in the first branch of the derived dendogram while the others were classified in the second branch of the dendogram. The average similarity between the two branches was 0.616. Among the primers tested, OPAB-03, 04, 05, 07, 09, 11, 14, 16, 18, and 19 showed polymorphic bands. Amplified fragments ranged from 200 to 1700 base pairs and the number of bands for each primer varied from 1 to10. The results obtained agrees well with those of Rize Tea Institute made by morphological characteristics.