Antibiotic resistance profile, serotyping and identification of Vibrio anguillarum isolated from rainbow trout (Oncorhynchus mykiss) cultured in cages in the Eastern Black Sea Region of Turkey, were performed in the study. The biochemical, serological and molecular techniques were used in identification of V. anguillarum isolates. The sequenced isolates were confirmed to be similar to V. anguillarum which stored under different accession numbers in GenBank database. The rate of 97-99% similarity were found among the compared isolates. All isolates were identified to be serotype O1 by slide agglutination test results. The antibiotic susceptibility test results showed that V. anguillarum isolates were resistant 100% to sulphamethoxazole, 90.6% to ampicillin, 71.9% to erythromycin, 62.5% to oxytetracycline, 46.9% to streptomycin and 31.3% to trimethoprim-sulfamethoxazole, but susceptible to oxolinic acid, enrofloxacin and florphenicol.