The biocontrol activity of Trichoderma atroviride ID20G against to biotic stress of Rhizoctonia solani B227 in bean development


ALPAY KARAOĞLU Ş., Arif B., Müberra P., Cemre T., Gökhan V.

1th International EurasiaMycology Congress to be held in Manisa, 3 - 05 July 2017

  • Publication Type: Conference Paper / Summary Text
  • Recep Tayyip Erdoğan University Affiliated: Yes

Abstract

The aim of the study was to determine the efficacy of Trichoderma atroviride ID20G strain isolated from tea soil on germination of seeds (tomato, maize and bean) and to investigate the biocontrol efficacy against biotic stress of Rhizoctonia solani B227(AG4) isolate in beans. The pathogenicity test against the bean development of the R. solani B227 and the efficiency of the T. atroviride ID20G strains for seed germination success were determined by the water agar method. In the presence of T. atroviride ID20G and R. solani B227, bean development parameters were investigated by pot experiment.  Physiological parameters (root and shoot length, number of hair root, fresh and dry weights, etc.) were measured and then statistical analyzes were performed.  It was determined that T. atroviride ID20G strain had no negative effect on the germination success of the seeds compared to the control. In the pot experiment, when the control  and R. solani groups were compared with each other, it was determined that there was a significant difference between the results of the statistical analysis performed with the shoot and main root length, leaf count, the number of lateral root, fresh and dry shoot weight and root weight (Tukey test, p<0.05).   T. atroviride ID20G was found to increase the length of the shoot and the number of lateral root and leaf, fresh and dry shoot weight and root weight more than the control.   In the presence of biotic stress, the administration of T. atroviride ID20G negatively affected the development of bean, whereas it was positively affected in the absence of biotic stress. It was concluded that T. atroviride ID20G strain could not be used as a biocontrol agent against R. solani pathogen but could be a plant growth promoting agent under healthy conditions. Keywords: T. atroviride ID20G, Rhizoctonia solani, biocontrol, plant pathogen