The effects of dexmedetomidine on early acute kidney injury in severely burned rats

ÖZDEMİR A., TOPÇU A., MERCANTEPE T., ARPA M., Karaka S., Ozdemir A. S., ...More

EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, vol.27, no.4, pp.1311-1321, 2023 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 4
  • Publication Date: 2023
  • Doi Number: 10.26355/eurrev_202302_31365
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, MEDLINE, Directory of Open Access Journals
  • Page Numbers: pp.1311-1321
  • Keywords: Burn, Dexmedetomidine, Inflammation, Kidney, Ox-idative stress, Rat, OXIDATIVE ORGAN DAMAGE, MODEL, TISSUE
  • Recep Tayyip Erdoğan University Affiliated: Yes


OBJECTIVE: Burns are a global medical and economic problem. In addition to high costs, the lengthy therapeutic process and the emotional trauma experienced by patients and their families indirectly worsen the socio-economic damage caused. Kidney failure observed after burns is highly correlated with mortality.MATERIALS AND METHODS: Twenty-eight male Sprague-Dawley rats (age four months, weight 250-350 g) were included in the study. They were randomly assigned into four groups consisting of seven rats each with similar mean weights. Group 1 (n=7) represented the healthy control group (C), Group 2 (n=7) the Sham+dex-medetomidine (DEX) 100 mcg/kg (three dos-es) (S+DEX100) group, Group 3 (n=7) the 30% Burn (B), and Group 4 (n=7) the 30% Burn+-DEX 100 mcg/kg/day group (B+DEX100) (three doses). Thiobarbituric acid reactive substanc-es (TBARS), total thiol (TT), interleukin-1 (IL -1) and tumor necrosis factor-alpha (TNF-alpha) values in kidney tissues were investigated biochemi-cally, and histopathological analyses were also performed. Nuclear factor kB (NF-kB)/p65 was measured using immunohistochemistry, and the TUNEL assay was applied to indicate apoptotic tubular epithelial cells.RESULTS: TBARS, IL-1, and TNF-alpha in kidney tissues decreased in the B+DEX100 group com-pared to the 30% burn group, while total thi-ol values increased. Histopathologically, atypi-cal glomeruli, particularly necrotic tubules, and inflammation in peritubular areas decreased in the B+DEX100 group compared to the 30% burn group. In addition, apoptotic tubular epithelial cells exhibiting TUNEL positivity and tubular ep-ithelial cells exhibiting NF-k beta/p65 positivity also decreased in the B+DEX100 group compared to the 30% burn group.CONCLUSIONS: Dexmedetomidine reduced apoptotic activity in rats and exhibited anti-in-flammatory antioxidant effects in the burn mod-el in this study.