Melatonin Prevents Radiation-induced Oxidative Stress and Periodontal Tissue Breakdown in Irradiated Rats with Experimental Periodontitis

KÖSE O., ARABACI T., KIZILDAG A., Erdemci B., Ozkal Eminoglu D., GEDİKLİ S., ...More

JOURNAL OF PERIODONTAL RESEARCH, vol.52, pp.438-446, 2017 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 52
  • Publication Date: 2017
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.438-446
  • Recep Tayyip Erdoğan University Affiliated: Yes


Aim: The aim of this study is to analyze the biochemical and histochemical effects of radiation therapy and protective melatonin administration on periodontal tissues in rats with experimental periodontitis.

Materials & Methods:60 male Sprague Dawley rats were divided 6 groups as follows; C: control , Ped: Experimental periodontitis group, Rt: Radiotheraphy administration group, PedRt: Rats with experimental periodontitis and exposed to irradiation, Rt-Mel: Radiotherapy and protective melatonin administration group, Ped-Rt-Mel: Periodontitis, radiation therapy and protective melatonin administration group. The rats were sacrificed at the end of the experimental procedure, and oxidative stress level and periodontal destruction were compared among the groups.

Results:OSI, 8-OHdG, MDA and CTX levels were found significantly higher in Ped-Rt gorup compared to Ped group(p<0.05), and the levels were found lower in Ped-Rt-Mel group with respect to Ped-Rt group(p<0.05). Alveolar bone destruction and attachment level were also found significantly lower in Ped-Rt-Mel group with respect to Ped-Rt group(p<0.05).

Conclusion:It was found that radiotherapy increased the oxidative stress, attachment level and alveolar bone loss, and protective melatonin administration significantly reduced the oxidative parameters and prevented periodontal damage in irradiated rats with experimental periodontitis. Further researches are needed for the use of systemic melatonin administration prior to radiation therapy.