Akademik Veri Yönetim Sistemi
Genetic Resources and Crop Evolution, cilt.70, sa.6, ss.1867-1877, 2023 (SCI-Expanded)
Cowpea belongs to the fabaceae family, originated in Africa, and grown in subtropical and tropical areas worldwide. Cowpea seeds contain high nutritional contents and play a vital role in nutritional feed for animals and humans in many countries. The present investigation aimed to check the genetic variations among cowpea germplasm collected from 6 countries using iPBS-retrotransposons markers system. Twelve highly polymorphic iPBS-retrotransposons primers were used for PCR amplification. These primers generated 200 total bands of which 188 were highly polymorphic and 94.30% polymorphism was observed. Mean values of genetic diversity indices i.e., Shannon’s information index (I = 0.452), effective number of alleles (ne = 1.501), overall gene diversity (ht = 0.236), gene diversity (h = 0.298), polymorphism information content (PIC = 0.308), and average genetic distance 0.61 confirmed a great level of genetic variations in studied germplasm. Analysis of molecular variance revealed 96% variation within the population. The STRUCTURE analysis divided germplasm into 2 populations according to geographically. The Neighbor-joining tree and Principle coordinate analysis (PCoA) divided the germplasm into 3 groups and the grouping was mostly aline with STRUCTURE-based clustering. This study revealed high genetic variability in cowpea germplasm and the high values of diversity indices revealed the utility and efficiency of iPBS-retrotransposons marker system. The PCoA, Neighbor-joining tree and STRUCTURE analysis separated the accessions geographically. Analysis of molecular variance revealed 94% genetic variation within a population and Ghana 1 and Turkey 4 had the greatest genetic distance and could be recommended for breeding activities.